帕米磷酸钠和降钙素对人工关节磨损微粒引起单核细胞分泌溶骨性因子的影响

Pharmacologic effects of pamidronate and calcitonin on aseptic poosening of prosthesis in vitro

目的 探讨帕米磷酸钠和降钙素防治人工关节松动的可能性。方法 采集人体外周血 ,分离单核细胞 ,分组培养。实验分 4组 ,第 1组 :仅单核细胞 ,为对照组 ;第 2组 :单核细胞及微粒 ,为微粒组 ;第 3组 :单核细胞、微粒及帕米磷酸钠 (阿可达 ) ,为阿可达组 ;第 4组 :单核细胞、微粒及降钙素 (密钙息 ) ,为密钙息组。培养 48h后 ,检测细胞上清液中肿瘤坏死因子 (TNF) α、白细胞介素 (IL) 1和IL 6的含量。结果 微粒组的细胞上清液中溶骨性因子含量明显高于对照组 (P <0 .0 1) ;而阿可达组和密钙息组的溶骨性因子含量明显低于微粒组 (P <0 .0 1)。结论 阿可达和密钙息能有效地抑制微粒刺激单核巨噬细胞分泌溶骨性因子 ,从而间接抑制破骨细胞的活性。

Objective To explore the possibility of pharmacologic agents inhibiting aseptic loosening of prosthesis induced by particles.Methods the human peripheral blood was collected and the peripheral monocytes (PBM) were separared.The monocytes were divided into four groups equally.Group1:monocytes only,as the control group;group2:monocytes+ the particles;group3:monocytes the particles pamidronate;group4:monocytes the particles calcitonin.Each group was cultured for 48 hours,and the amount of osteolytic factors such as TNF-α,IL-1 and IL-6 of each group was determined.Results It was found that the levels of these factors were much higher in group 2 than those in group 1,3 and 4 (P< 0.01).Colusion these particles can stimulate monocytes to excrete osteolytic factors,which can be inhibited effectively by bisphosphonates and calcitonin.Pamidronate and calcitonin may have clinical utility in the prevention and treatment of aseptic loosening of prosthesis.