大鼠移植动脉硬化加快模型的建立

Promoted transplant arteriosclerosis in a rat aortic model

目的 建立一种简捷 ,有代表性且稳定的移植物动脉硬化模型。方法 将SD大鼠的腹主动脉分别冷缺血 1、2 4、48h行SD→SD及SD→Wistar的原位腹主动脉移植 ,观察术后植入段血管病理改变、TGF β1表达及手术前后过氧化脂质的变化。结果 SD→SD及SD→Wistar缺血1h组分别于术后 10周及 6周见内膜明显增厚 ,而缺血 2 4h组只需 2周 ;各组移植后 2h过氧化脂质均明显高于术前 ,术后 4、2 4h与术前比差异无显著性 (P >0 .0 5 ) ;强化缺血组TGF β1不论是SD→SD还是SD→Wistar均于术后 1周即出现高表达。结论 以SD/Wistar作为供 /受体行腹主动脉移植 ,强化冷缺血损伤 ,可加快移植物动脉硬化 ,可望成为新型慢排模型。

Objective To establish a novel,representative and steady transplant arteriosclerosis model.Methods Abdominal aorta grafts from SD rats were cold stored for 1,24,or 48 h before being orthopically transplanted to SD or Wistar recipients.The pathohistological changes and the expression of TGF-β1 of the grafts were observed.The changes of serum lipid peroxides pre- and post-transplantation were also measured.Results The intimal thickness of SD→SD and SD→Wistar ischemia for 1 h was significantly greated than that of pre-transplantation in 10 weeks and 6 weeks respectively,whereas grafts with 24 h of ischemia developed pronounced thickening in 2 weeks.Serum levels of lipid peroxides were significantly higher in 2 h post-transplantation in all groups than pre-transplantation,but there was no significant different pre- and 4 or 24 h post-transplantation.The staining intensity of TGF-β1 in 24 and 48 h of ischemia was stronger one week after transplantation regardless of syngeneic or allogeneic transplants.Conclusion The transplantation of abdominal aorta grafts used SD/Wistar as donors and recipients with prolonged cold ischemia time is sufficient to promote the development of transplant arteriosclerosis.