顺铂对人舌癌细胞的抑制作用和对端粒酶活性的影响

The inhibiting effect of cisplatin on cells and telomerase activity of squamous cell carcinoma of tongue

目的 研究化疗药物顺铂对人舌癌Tca8113细胞的抑制作用和对端粒酶活性的影响及其抗癌机制 ,并初步探讨端粒酶作为化疗敏感性指标的可行性。方法 应用顺铂处理的人舌癌Tca8113细胞为用药组 ,相同条件培养但不用药的细胞为对照组。MTT法研究细胞的生长和增殖情况 ,双层琼脂培养法研究细胞的克隆形成率 ,透射电镜观察细胞的超微结构 ,应用流式细胞术检测细胞周期 ,TRAP PCR ELISA法检测人舌癌Tca8113细胞端粒酶活性。结果 顺铂对人舌癌Tca8113细胞有明显的抑制作用 ,且呈浓度依赖性 ;能明显改变细胞大体形态和超微结构 ;抑制端粒酶活性且有一定的时间依赖性 ;细胞周期改变不明显。结论 顺铂可以明显抑制Tca8113细胞的增殖活性及其转移复发倾向 ,还可作用于线粒体 ,抑制端粒酶活性 。

Objective To study the inhibiting effect of cisplatin on human tongue Tca8113 cells and telomerase activity.Methods Tca8113 cells were treated in vitro with cisplatin and the cells untreated with cisplatin as controls.The inhibiting effect and the chang of telomerase activity induced by cisplatin in human tongue cells Tca8113 were detected by MTT assay in vitor,clonogenic assay,transmission electron microscope,flow cytometry analysis techinique and TRAP?PCR?ELISA.Results Activity of Tca8113 cells treated with cisplatin was significantly inhibited,that was density-dependent.Morphology of cell was changed but cell cycle was not changed.Telomerase activity of Tca8113 cells treated with cisplatin was significantly inhibited,and the effect was time-dependent.Conclusions Cisplatin can effectively inhibit the cell activity and inclination of transfer and recurrence of Tca8113 cells.The cispiatin can act on the mitochondrions of cells.It can refrain the telomerase activity and change the cell cycle little at the same time.