染料木黄酮诱导膀胱癌T24细胞凋亡的实验研究

Genistein induces apoptosis in human urinary bladder cancer T24 cells

目的 研究染料木黄酮对人T2 4膀胱癌细胞的抑制作用和诱导凋亡作用 ,并探讨其作用机制。方法 将不同浓度的染料木黄酮作用于人T2 4膀胱癌细胞 ,用MTT法检测其有效作用浓度 ,分别采用透射电子显微镜、瑞氏染色、Hoechest332 5 8荧光染色、流式细胞仪观察和检测T2 4细胞凋亡情况。结果 MTT法测得染料木黄酮对T2 4细胞的IC50 值为32 80mg·L-1,浓度为 2～ 80mg·L-1的染料木黄酮具有诱导人T2 4膀胱癌细胞凋亡的作用 ,且随药物作用时间延长凋亡率逐渐增加。结论 染料木黄酮具有抗膀胱癌作用 ,其重要作用机制之一是诱导人T2

Objective To study the inhibitory effect of genistein on human urinary bladder cancer T24 cells and its mechanism.Methods MTT assay was used to identify the effect of genistein on human urinary bladder cancer T24 cells in vitro.The cells apoptosis was detected by electron micrographs,Wright's staining,Hoechest33258 fluorescent staining and flow cytometry.Results The IC 50 value of genistein for T24 cells was 32.8?mg·L -1.The apoptotic cells were induced by genistein at the concentration of 2～80?mg·L -1.Conclusions Genistein can inhibit human urinary bladder cancer T24 cells and induce apoptosis at some concentrations.The induction of apoptosis is a very important mechanism of genistein to treat urinary bladder cancer.