固定HCG抗体膜的跨膜电位

THE TRANSMEMBRANE POTENTIAL OP HCG ANTIBODY-BINDING MEMBRANE

高分子膜作为一项新兴技术,在很多领域得到日益广泛的应用.近十几年,随着生物工程和生物传感器的迅速发展,高分子生物功能膜的研究倍受重视.高分子生物功能膜是采用固定化技术,将具有分子识别功能的材料(如酶、抗原、抗体等)固定在高分子膜上而制得的.在固定化膜表面发生的生物化学反应,可以引起膜的荷电状态的变化,从而导致跨膜电位的变化。有关固定化膜的报导较多,但主要限于固定化的方法及其应用方面的研究。

In this paper the antibody of HCG was immobilzed on the cellulose acetate membrane. The membrane potentials varied with the electrolyte concentrations of the two aqueous phases. The charge density of the membrane phase was lowered asthe immunochemical reaction of HCG with HCG antibody proceeded on the membranesurface. When c_1/c_2=8.0, the antibody-bound membrane potentials shifted to posi-tive values with the increase of HCG concentrations. The membrane immunosensorfor the measurement HCG in the solution was constructed according to the change ofthe membrane potential. A linearity between Δφ and lg HCG can be obtained in theconcentration range of 50--2500 mlU·ml^(-1) HCG.