增生性玻璃体视网膜病变增生膜中survivin基因的表达

Expression of survivin gene in periretinal membranes of proliferative vitreoretinopathy

目的 :探讨凋亡抑制基因survivin在增生性玻璃体视网膜病变 (PVR)增生膜中的表达 .方法 :玻璃体手术中取出的 10例PVR患者的增生膜 ,应用免疫组织化学方法(SABC法 )检测增生膜中survivin基因的表达 .结果 :10例增生膜中 ,6例表达阳性 .阳性染色位于膜上大部分细胞的胞质内及细胞外基质中 .结论 :Survivin基因的异常表达引起细胞的凋亡抑制 。

AIM: To study the expression of survivin, an inhibitor gene of apoptosis, in periretinal membrane of proliferative vitreoretinopathy (PVR). METHODS: 10 periretinal membranes obtained from 10 cases of PVR with retinal detachment undergoing vitretomy were used to examine the expression of survivin gene by SABC method. RESULTS: Survivin gene was expressed in 6 of the 10 cases. The reactions of survivin were observed in the ctyoplasm and extracellular matrix. CONCLUSION: The abnormal expression of survivin gene inhibits apoptosis and it may participate in the progression of proliferative vitreoretinopathy.