无精子症患者精子生成基因及一氧化氮水平的探讨

Spermatoleosis gene and NO level in patients with azoospermia and oligozoospermia

目的 :探讨非梗阻性无精子症和严重少精子症患者血中精子生成基因 (AZF)及一氧化氮 (NO)的关系 .方法 :AZF分析 :采用多重聚合酶链反应扩增技术检测其特异性基因位点的缺失情况 ;NO用镀铜镉还原荧光法 .检测NO代谢产物硝酸盐 (NO-3 ) .结果 :5 0例无精子症和严重少精子症中有 5例患者显示AZF不同区域片段的缺失 (AZFa sY86 ,AZFb sY12 7和AZFc sY2 5 4各 1例及AZFc sY2 5 5缺失 2例 ) ,发生率为 10 % ,而生育对照组无 1例缺失 .AZF缺失者NO水平明显高于未缺失者和对照组 ,分别为 (12 8.2 6±18.5 1) ,(85 .6 8± 2 6 .5 6 )和 (35 .6 8± 18.5 6 ) μmol·L-1,三者之间有显著差异 (P <0 .0 1和 0 .0 0 1) .结论 :AZF基因位点的缺失和男性无精子症和严重少精子症有一定的关系 ,可以明确病因 ,减少一些无谓的治疗 ,采用助孕技术后代也有可能存在不育 .过量的NO对机体细胞产生损害 。

AIM: To discuss the relationship between the azoopermia factor (AZF) and the level of nitric oxide (NO) in patients with unobstructed azoospermia and severe oligozoospermia. METHODS: The deletion of AZF gene locus was analyzed by PCR and the metabolic product (NO - 3) of NO was measured by copper coated cadmium reduction method. RESULTS: The deletion of AZF fragments (AZFа sY86, AZFь sY127, AZFс sY254 and AZFс sY255) was found in 5 cases of the 50 patients with azoospermia and severe oligozoospermia, while the deletion of AZF fragments was not detected in control group. The level of NO in AZF deletion patients (128.26±18.51) μmol·L -1 was significantly higher than that in no deletion patients (85.68±26.56) μmol·L -1 and control group (35.68±18.56) μmol· L -1 ( P <0.01 ). CONCLUSION: There is some relationship between the deletion in AZF gene and azoospermia and severe oligozoospermia and excessive NO is harmful to germ cells, thus affecting the reproducibility.