利用Tet-OnTM基因表达系统直接克隆 p53下游基因(英文)

Direct cloning of p53 downstream genes using Tet-On^(TM) gene expression system

目的 直接克隆 p5 3下游基因 ,并对其功能进行初步研究。方法 采用哺乳动物细胞诱导表达系统 -Tet-onTM基因表达系统 ,建立 p5 3基因诱导表达可调控的细胞系 ;通过mRNA差异显示技术直接克隆p5 3下游基因 ,并利用原位杂交技术检测其在小鼠胚胎发育过程中的表达。结果 克隆到一个新的p5 3下游基因侯选基因 ,并检测到它在小鼠胚胎发育过程中有特异性表达。结论 直接克隆 p5 3下游基因的成功 ,为进一步研究 p5 3基因的功能奠定了基础。

Objective: To clone p53 downstream genes directly and study their functions.Methods:A mammalian gene inducible expression system, Tet-OnTM Gene Expression System was used, and the regulable cell line of the inducible expression of p53 gene was established; mRNA difference display technique was used to clone p53 downstream genes directly and in situ hybridization technique was used to initially understand their functions in mouse embryonic development. Results:A novel p53 downstream gene was gained and its specific expression in the course of mouse embryonic development was observed. Conclusion: Direct cloning of novel p53 downstream genes can provided basis for an in-depth study on p53 functions.