雷竹笋汁对四氯化碳致大鼠急性肝损伤的防治作用

The Prophylactico-therapeutic Effect of Leizhusun Juice on the Liver Injury Induced by Carbon Tetrachloride in Rats

目的 :探讨雷竹笋汁对肝损伤的防治作用。方法 :分别对健康大鼠和预先用四氯化碳制作的肝损伤模型大鼠用不同剂量的雷竹笋汁 (Ⅰ组 2 0 5.70mg·kg 1,Ⅱ组 43 6.40mg·kg 1)灌胃 7d ,然后将健康大鼠亦用四氯化碳制备肝损伤模型 ,并另取健康大鼠作空白对照 ,测定各组大鼠血清丙氨酸氨基转移酶 (ALT)、天冬氨酸氨基转移酶 (AST)和超氧化物歧化酶 (SOD)活性及丙二醛 (MDA)含量。结果 :雷竹笋汁显著降低实验大鼠血清ALT和AST的活性 ,升高SOD活性及降低MDA含量 ,且呈量效关系。结论 :雷竹笋汁对四氯化碳致大鼠急性肝损伤有明显的防治作用 。

Objective:To probe into the prophylactico-t he rapeutic effect of leizhusun juice (LZS) on the experimental liver injury in rats. Methods:44 wistar rats were randomly divided into four g roups. 8 animals of group 1 served as blank controls. Rats of group 2,3 and 4(wi th 12 animals in each group) were given each by gastrogavage normal saline, 205. 70 mg·kg -1 of LZS and 436.40 mg·kg -1 of LZS in equal volumes res pectively, q.d. for 7 consecutive days. After the last medication, each animal o f group 2,3 and 4 was given an intraperitoneal injection of 0.1 mL/100 g body we ight of 50% CC1 4 in peanut oil. 72 hours later blood was taken from each of th e 8 surviving animals of the four groups and serum separated for the determinati on of the activities of alanine aminotranferase (ALT), aspartate aminotranferase (AST) and superoxide dismutase (SOD) as well as the content of malonyldialdelyd e (MDA). Results:CC1 4 was shown to cause a sharp rise in the se rum levels of ALT, AST and MDA and a striking fall of the serum SOD level in ani mals of group 2. In rodents of group 3 and group 4 that had been pretreated with two different doses of LZS before CC1 4 poisoning the serum levels of ALT, AST and SOD were significantly lower while the serum content of MDA was significant ly higher than those of the animals of group 2 (P<0.05,P<0.01). A dose -effect relationship of LZS was also demonstrated. Conclusion:LZS was shown to have a distinct prophylsctic o-therapeutic effect directed against acute liver injury induce by CC1 4 in ra ts, the underlying mechanism maybe related to its effect of antioxidation.