肺耐药蛋白和增殖细胞核抗原在骨肉瘤中的表达

EXPRESSION OF LUNG RESISTANCE PROTEIN AND PROLIFERATIVE CELL NUCLEAR ANTIGEN IN OSTEOSARCOMA

目的 :探讨肺耐药蛋白 ( LRP)、增殖细胞核抗原 ( PCNA)在骨肉瘤组织中的表达及其与多药耐药 ( MDR)之间的关系。方法 :用免疫组织化学的方法检测了 42例石蜡包埋的骨肉瘤组织和 1 6例正常骨组织中 LRP、PCNA的表达。结果 :42例骨肉瘤中 LRP、PCNA的表达率分别为 42 .86%( 1 8/42 )、95 .2 4 %( 4 0 /42 )。正常骨组织中无 LRP的表达 ,PCNA的阳性率为 6.2 5 %( 1 /1 6)。这两种指标的表达无相关性 ( P>0 .0 5 ) ;LRP和 PCNA的表达在不同的分型之间无显著性差异 ( P>0 .0 5 )。对 2 7例患者进行了随访 ,LRP阳性 1 5例 ,LRP阴性 1 2例。其 2年、5年的生存率分别为 40 %( 6/1 5 )、、6.67%( 1 /1 5 )和 75 %( 9/1 2 )、33.33%( 4 /1 2 )。结论 :骨肉瘤预后差与 LRP的表达有关 ,LRP的表达和

Objective:To investigate the expression of lung resistance protein(LRP) and proliferative cell nuclear antigen (PCNA).Methods:Expression of LRP and PCNA was immunohistochemically studied in paraffin sections from 16 normal bone tissues and 42 specimens of osteosarcoma from patients who had received no chemotherapy before surgery.Results:42 86% (18/42) LRP and 95 24% (40/42) PCNA were positive in osteosarcomas,in normal bone tissues LRP were negative,but the positive rate of PCNA was 6 25%(1/16).Among 27 followed up cases,15 were LRP negative,2 and 5 years survival rates were 75%(9/12) and 16 67% (2/12).Conclusion:Low survival rate of osteosarcoma is associated with LRP.The expression of LRP is not associated with that of PCNA.