孕期干预阻断乙肝病毒母婴垂直传播效果评价

Preventive effect of the vertical transmission of hepatitis B virus demonstrated by seroassays for viral transcripts

目的 探讨孕期干预治疗阻断乙型肝炎病毒母婴垂直传播的效果。方法 将 6 0例 HBs Ag/ HBe Ag阳性孕妇分成治疗组 31例 ,对照组 2 9例。治疗组均在孕 2 6周起开始注射乙肝免疫球蛋白 (HBIG)及外用左旋咪唑涂布剂 ,采用 EL ISA法检测孕妇和新生儿血清 HBs Ag、HBAb、Hbe Ag、HBe Ab、HBc Ab,用 PCR电泳和 RT-PCR法测定 HBV- DNA及全长型和顿挫性转录体。分析母婴之间乙肝病毒分子水平上的关系。结果 新生儿外周血中 HBs Ag阳性者 2例 ,宫内感染率为 6 .4 5 %。对照组 4例 ,宫内感染率 13.7%。与对照组比较差异有显著性。 (P<0 .0 1) ,两组都有超过一半的患儿携带顿挫型病毒转录体。结论 携带 HBV孕妇于孕晚期给 HBIG和左旋咪唑涂布剂联合治疗后 ,可有效降低婴儿 HBs Ag和 HBV-

Objective To probe into prevent methods on mother-infant vertical transmission of Hepatitis B virus(HBV).Methods 60 cases chronic HBsAg-carriers pregnant women were divided into two groups.31 cases were injected with Hepatitis B virus immunoglobulin and levamisole liniment during pregnancy since the 26th week of gestation;there were 29cases without injection of HBVac or HBIG and other.Serologic HBV markers( HBsAg,HBsAb,HBeAg,HBeAb and HbcAb) of pregnant women and newborns umbilical blood were detected by ELISA.The serum viral nucleic acids were analyzed using PCR and RT-PCR.Results HbsAg positive rate of newborns umbilical blood were 6.45% and 13.7% with different treatment,respectively. Conclusion Immunization with immunoglobulin and levamisole liniment during pregnancy may prevent intrauterine HBV infection effectively.