单宁酸-氯化铁法媒染肺内微血管及细胞的组织学观察

Observation of Pulmonary Microvasculature and Cells with Tannic Acid-Ferric Chloride(TA-Fe) Staining under the Light Microscope

目的 光镜下观察大鼠肺微血管及肺组织细胞。方法 应用单宁酸 氯化铁法 (TA Fe法 )灌流固定大鼠 ,取肺做冰冻切片 ,氯化铁显色 ,光镜下观察。结果 肺内小血管、微血管蜿蜒走行 ,被切成各种断面 ,管壁平滑肌细胞、内皮细胞显示清晰 ,有一定立体感 ;接近被膜的微动脉形成较粗的毛细血管 ,肺泡间毛细血管不易分辨 ,肺间质内有微血管和毛细血管分布 ,微血管周围的肺组织亦被媒染。结论 TA Fe法即可显示肺内微血管 ,又能保存组织细胞结构 ,肺内小动脉和微动脉管壁的平滑肌参与微循环的主动调节机制。

Objective To observe the pulmonary microvasculature and cells in the rats under the light microscope. Methods After the rats were perfused with compound staining fixative solution, the lung was cut into slices and immersed in 2% ferric chloride solution and observed under light microscope. Results The small blood vessels and capillaries of the lung were in wriggled way and cut in different cross sections. The smooth muscle cells and the endotheliocytes in the vessles wall could be clearly observed in distinct three dimensions. The capillaries arose from arterioles near serosa were relatively thick. It was uneasy to discern the capillaries in between the pulmonary alveolus. More arterioles and capillaries are presented in the pulmonary interstitium. The pulmonary tissue around arterioles was also stained. Conclusion It is proved that TA Fe method is a convenient technique in visualizing microvasculature in lung but also in keeping tissue structure. The smooth muscles in the wall of pulmonary small arteries and arterioles may be involved in active adjustment mechanism in microcirculation.