黑色素生物合成抑制剂的初步研究

Primary Studies on Melanin Biosynthesis Inhibitors

利用比基尼链霉菌筛选模型和急性细胞毒性试验从 170 0株放线菌中筛选出编号为MBI 3 9-2 4的菌株作为黑色素生物合成抑制剂研究的试验菌株 ,并初步鉴定为秃裸链霉菌 (Streptomycescalvus)。借鉴了一些天然药物的提取和分离方法 ,分离出 4种具有抑制黑色素生物合成活性的单峰物质 ,并将其对B16鼠黑素瘤细胞中黑色素合成的抑制效果进行了评价 ,确定了抑制效果最好的单峰物质MBI -3 # ,其对于B16鼠黑素瘤细胞中黑色素合成的抑制活性为 15 μg/mL ,细胞毒性与最小抑制浓度的比值为 16 67。

The Streptomyces bikiniensis screening model and the experiment of acute cell toxicity were used to screen melanin biosynthesis inhibitors. The strain of Streptomyces sp. number N39 24 (named as MBI 39-24) was chosen as the experimental strain for the research of melanin biosynthesis inhibitor from 1700 actinomycetes. It was identified as Streptomyces calves. Four kinds of material with mono peak that had the melanin inhibiting bioactivity were separated adopting natural medicine extraction methods. The four kinds of material with mono peak were tested for inhibitory effect on melanin synthesis in cultured melanin producing murine B16 melanoma cells. MBI 3 # was proved to be the most effective through the inhibition comparison and the cell toxicity/inhibition ratio on melanin synthesis. Its inhibitory bioactivity on melanin synthesis in cultured melanin producing murine B16 melanoma cells was 15 μg/mL. The ratio of cell toxicity and inhibition on melanin synthesis was 16.7.