银杏黄酮的酶法提取工艺研究

Study on Extraction Process of Flavone from Ginkgo biloba Leaves by Enzymic Treatment

本文研究了银杏黄酮的酶法提取工艺,银杏叶原料经纤维素酶预处理后浸提,总黄酮得率显著提高,得率可达到2.01％。其酶解过程的最优参数为:料液中酶浓度为0.125g/L,酶与底物配比为1:1200,酶解温度45℃,自然pH值,酶解时间2 h。

The extraction technology of flavonoids from Ginkgo biloba leaves was studied in this paper. When the crude leaves used the cellulase enzyme pretreatment before extraction process, the extraction yield of total flavone was up to 2. 01%. The extraction conditions were also determined experimentally as following: concentration of enzyme in the liquor being 0. 125 g/L, mixture ratio between enzyme and substrate being 1:1200, treating 2 hours, at 45℃ , under natural pH value.