鸡肝散总黄酮抗胸痹证的实验研究

Experimental Research on Total Flavone from Elsholtzia blanda against Xiongbi Symptom

目的:研究鸡肝散总黄酮(Total flavone from Elsholtzia blanda,TFEB)抗冠脉结扎诱发大鼠胸痹证之功效及其机制。方法:雄性SD大鼠ig TFEB5天,结扎冠脉左前降支(left anterior descending coronary artery,LAD)造成大鼠胸痹模型,3 h后处死大鼠,取左心室,氯化硝基四氮唑蓝(nitrotetrazolium blue chloride,NBT)染色以区分梗死区和非梗死区,计算心肌梗死指数;另取心室肌心尖部分做组织切片,HE染色,光镜检查心肌的损伤程度,评价TFEB对胸痹证的作用。取血清测定一氧化氮(Nitric oxide,NO),探讨TFEB抗胸痹证的机理。结果:TFEB各剂量组均可著减少大鼠冠脉结扎后左室梗死区的心肌重量,心肌梗死指数明显减小(P<0.05～0.01),呈良好的剂量依赖关系。光镜检查结果显示,假手术组心肌形态学无明显改变,肌纤维完整,心肌细胞排列整齐;溶剂组心肌纤维大范围肿胀、断裂、坏死融合,并可见心肌细胞破裂;TFEB各组心肌病理性变化程度都较溶剂组减轻。TFEB100、150 mg/kg均可显著降低大鼠冠脉结扎后血清中NO含量(P<0.05～0.01)。结论:TFEB能对抗大鼠冠脉结扎诱发的心肌缺血,具有对抗胸痹证之功效,其机制可能与TFEB降低血清NO含量有关。

Objective:To study the protective effect and mechanism of total flavone from Elsholtzia blanda (TFEB) on Xiongbi symptom induced by coronary artery ligation in rats. Methods: The left anterior descending coronary artery (LAD) of male rat was ligated for 3 h after feeding TFEB or 0. 5% CMC for 5 d by ig. Then, the rats were decollated and the left ventricle were dyed by nitrotetrazolum blue chloride (NBT) to distinguish infraction area from non-infraction area. Myocardial infarction index was calculated accordingly to assess the effect of TFEB on myocardial ischemia. A piece of ventricular muscle come from apex of heart was dyed by hematoxylin and eosin ( HE) to observe the degree of myocardial lesions by microscopic examination. Nitric oxide (NO) level in serum was measured to demonstrate the relevant mechanism. Results:TFEB could markedly reduce the myocardial infraction index in a dose-dependent fashion (P<0.05-0.01). Microscopic examination showed that the myocardium of sham group had no lesion in morphology and the muscle fiber kept integrity and the cardiac myocyte lined up in order. While control group displayed extensive swelling, rupture, confluent, even break in cardiac myocyte. TFEB could relieve the lesion degree of myocardium. NO level in serum after LAD ligation in TFEB 100 and 150 mg/kg groups was markedly reduced( P <0. 05 -0. 01). Conclusion:TFEB has protective effect on myocardial ischemia induced by LAD ligation in rats and can prevent against Xiongbi symptom, possibly via the decrease of NO level in serum.