蕨菜黄酮类化合物的提取及其抗氧化作用

Study on the Extraction of Flavonoid Compound in Pteridium aquilinum (L.) and It's Antioxidant Property

用体积分数为 70 %的乙醇提取蕨菜黄酮类化合物 ,得到粗黄酮 ,经聚酰胺纯化 ,得到精制黄酮。定量测定黄酮含量 ,并对蕨菜黄酮类化合物抗氧化性进行了研究。结果表明 ,蕨菜总黄酮含量为干重的 7.2 8% ,粗黄酮中黄酮含量为 2 7.0 3 % ,精制黄酮中的黄酮含量为41 5 2 % ;蕨菜黄酮类化合物有较强的清除自由基能力 ,能显著阻断亚油酸、猪油的自氧化作用 ,表现出很强的抗氧化活性。

Raw flavonoid was extracted from Pteridium aquilinum (L.) by using 70% alcohol, followed by purification with polyamide to obtain refined flavonoid. The amount of pure flavonoid was quantified and its antioxidant property was studied in this research. Results show that the total flavonoid content in Pteridium aquilinum (L.) is 7.28% of the dry weight while pure flavonoid content represents 27.03% of raw flavonoid and 41.52% of refined flavonoid. Study also shows that the flavonoid in Pteridium aquilinum (L.) has strong capacity for scavenging free radicals, and can effectively inhibit linoleic acid and lard autoxidation, therefore exhibiting high antioxidant activity.