山楂黄酮的薄层色谱分离鉴定研究

A STUDY ON SEPARATION AND IDENTIFICATION OF FLAVONOIDS IN CRATAEGUS PINNATIFIDA BGE.BY THIN-LAYER CHROMATOGRAPHY

研究了山楂(山里红CrataeguspinnatifidaBge.var.majorN.E.Br)黄酮类化合物新的薄层色谱分离鉴定方法。以十二烷基硫酸钠 正丁醇 正庚烷 水微乳液为展开剂,利用聚酰胺薄层色谱法能使山楂黄酮完全分离。从山楂叶中分离得到7个黄酮斑点,山楂果中得到7个黄酮斑点,但二者的斑点不完全相同。与用正丁醇∶乙酸∶水4∶1∶5为展开剂的聚酰胺薄层色谱法比较发现,微乳薄层色谱法检测灵敏度高、分离效果理想。

This paper studied new method of separation and identification of flavonoides in Crataegus pinnatifida Bge.var.major N.E.Br.Influence of microemulsion types on power of resolving flavonoids was investigated with (SDS/n-C4H9OH/n-C7H(16)/H2O) microemulsion as eluant on polyamide film.The flavonoids were separated entirely.Seven flavonoids spots were obtained respectively from leaves and fruit,but they are not identical.Compared with the general mobile phase composed of C4H9OH∶CH3COOH∶H2O=4∶1∶5 the sensitivity of detection was improved markedly and the results were satisfactory.This new chromatographic method is simple,accurate for separation and identification of flavonoids in C.pinnatifida Bge.var.major N.E.Br.