混合线性分析法同时测定安痛定注射液中三组分含量

Simultaneous Determination of Three Components in Antongding Injection with Hybrid Linear Analysis

将一种新近发展的多元校正方法———混合线性分析法 ,用于安痛定注射液中氨基比林、安替比林、巴比妥含量的同时测定。在 2 2 0～ 2 5 0nm内取 1 6个波长测量 7组标准混合溶液的吸光度矢量作为校正集 ,以最小二乘法求得系数矢量作为各组分的纯光谱 ,用混合线性分析法进行数据处理。氨基比林、安替比林、巴比妥的标准回收率分别为 99.0 %～ 1 0 0 .3 %、99.0 %～ 1 0 0 .8%和 98.5 %～ 1 0 1 .1 %。用于安痛定注射液中氨基比林、安替比林、巴比妥三组分含量的测定 ,经t检验 ,其测定结果与标准法相比 ,两者间无显著性差异。

A newly developed multivariate calibration method-hybrid linear analysis was applied to determine aminopyrine, antipyrine and barbitone in antongding injection without separation. The absorbance matrix of 7 standard mixtures at 16 measuring points ranged from 220 to 250 nm was used for calibration. The pure spectrum of each component was obtained by least squares method. The recoveries of aminopyrine, antipyrine and barbitone for the analysis of synthetic samples were 99.0% similar to 100.3%, 99.0% similar to 100.8%, 98.5% similar to 101.1% respectively. This method has been successfully applied to the determination of aminopyrine, antipyrine and barbitone in antongding injection samples. The. comparison was also made with the standard method. The t-test showed that there was no significant difference between the results of hybrid linear analysis and standard method.