高效液相色谱指纹图谱应用于地黄的研究

Studies on the Application of High Performance Liquid Chromatographic Fingerprint to Rehmannia Glutinosa Libosch

利用高效液相色谱建立了地黄的色谱指纹图谱。采用反相C1 8柱 (5 μm ,1 5 0mm× 4.6mm) ,流动相甲醇∶水 (V V =5∶95 ) ,检测波长为 2 80nm ,流速 1 .0mL min进行实验。根据相对保留值和相对面积值对色谱指纹图谱进行分析对比研究 ,建立了控制地黄药材质量的新方法。该法为中药样品的鉴定提供了较全面的信息 ,并为HPLC在复杂组分的样品分析领域开拓了新的应用途径。

The chromatographic fingerprint of Rehmannia glutinosa libosch. is established by high performance liquid chromatography. The testing method involves a RP-C-18 column, a mobile phase of CH3OH : H2O (V/V = 5: 95), an ultraviolet detector at 280 nm and the flow rate 1.0 mL/min. According to the relative retention values and the relative area in chromatographic fingerprint, their comparative study is achieved. It established a new method of chromatographic fingerprint for Rehmannia glutinosa libosch.,, a traditional Chinese medicine. This method provides useful information for quality control of the material medica of traditional Chinese medicine.