螺旋纤维床固定化反应器两步法生产壳聚糖酶

Chitosanase Production in Convoluted Fibrous Bed Bioreactor with Two-Step Process

采用了菌体生长与产酶分步的新工艺利用里氏木霉 (Trichodermareesei)ATCC5 6764生产壳聚糖酶 ,酶活力比在相同条件下进行的一步法产酶提高了 1 7倍。采用此工艺在螺旋纤维床生物反应器中进行产酶试验 ,酶活比采用游离细胞培养又提高了 39% ,达到 0 2 4 6U mL。固定化菌丝还能够长期保持活性 ,在重复分批操作中 ,经过 1 0批共 1 5天的产酶实验 ,平均每批的酶活保持在 0 2 35U mL左右。

Chitosanase activity can be increased remarkably by a two-step process, in which, the growth of T. reesei was in the first step with glucose as carbon source and enzyme induction by D-glucosamine in the second step. With the novel process chitosanase activity produced was about 100% higher than that in the traditional one step process. Under the optimized culture conditions: aeration rate was 4L/min(2 vvm) and age of mycelia were 72 h, The activity of chitosanase produced by immobilized T. reesei in convoluted fibrous bed bioreactor(CFBB) was 246mU/mL which was about 39% higher than that with free cell culture in 5L CSTR under the same culture conditions. Furthermore, the novel bioreactor was also feasible to be operated in repeated batch mode. During 10 repeated-batch in 15 days, the average production of chitosanase activity to each batch was kept 235mU/mL.