几个水稻株系的亲和性测定及微卫星标记分析

Compatibility test and molecular marker analysis of rice lines

利用典型的籼稻南京11号、典型的粳稻秋光对16个水稻株系的亲和性进行了测定,测交结果表明,NL173、NL15、NL61、NL67、NL82和NL279表现亲籼性,NL208表现亲粳性,NL78表现一定广亲和性,其余株系与籼、粳测验种杂交育性都不高。根据测交结果选择不同育性的株系用分布于12条染色体上的前人证明籼粳稻间有多态性的56个微卫星标记进行分析,在微卫星标记聚类分析基础上计算粳型性判别值Dj,并据此把NL173、NL15、NL78、NL58判别为偏籼型,而NL208为偏粳型。综合亲和性测交结果和分子标记分析结果发现,在亲和性测交中表现亲籼的2个株系NL173、NL15分子标记判别为偏籼类型,杂交亲和性表现亲粳的株系NL208分子标记判断为偏粳类型,表现广亲和特性的株系NL78被分子标记判别为偏籼类型,与测验种杂交育性均不高的NL58分子标记判别为偏籼型。

To test the hybrid compatibility of some rice lines,two typical indica and japonica varieties,Nanjing 11 (indica) and Akihikari (japonica),were used as testers to cross with 16 rice lines.Based on the spikelet fertility of F1 hybrids,the 16 lines were classified into three different groups.NL15,NL61,NL67,NL82 and NL279 are indica-compatible,NL208 is japonica-compatible,and NL78 is wide-compatible.Two testers and 5 lines were investigated using the indica-japonica polymorphic microsatellite markers distributing over the 12 chromosomes.Obvious difference in the degree of indica-japonica differentiation among testers and lines were observed.Based on Dj values (japonica discrimination value),NL78,NL58,NL15,and NL173 were classified to indicaclinous group,and NL208 to japonicaclinous group.