p53、增殖细胞核抗原和Bcl-2在皮肤外毛根鞘癌中的表达

Expression of p53,PCNA and Bcl-2in Trichilemmal Carcinoma of Skin

目的探索外毛根鞘癌的临床病理学特征及p53、增殖细胞核抗原(PCNA)和Bcl-2的表达与意义.方法应用HE染色观察和免疫组化S-P方法检测22例外毛根鞘癌p53、PCNA和Bcl-2蛋白表达.结果22例外毛根鞘癌患者中女15例,男7例,年龄40～79岁,发生于头顶枕部皮肤16例.免疫组化标记结果显示:p53阳性表达为72%(16/22),阳性分布广泛;PCNA全部阳性,但阳性细胞在高中低分化中呈递增趋势;Bcl-2阳性为63%,在各级之间表达差异有显著性(P＜0.01).结论分叶状结构和骤然角化是外毛根鞘癌的组织学特征;p53、PCNA、Bcl-2在外毛根鞘癌均有不同程度的表达,参与肿瘤发生发展和生长调控.

Objective To investigate the clinicopathologic features and the expression of p53,PCNA and Bcl-2in tricholemmal carcinoma.Methods Skin lesions were studied with HE staining and immunohis-tochemical markers for p53,PCNA and Bcl-2(S-P method)in22cases of skin tricholemmal carcinoma.Both morphological manifestations and immunohistochemical expression were observed.Results There were15fe-male and7male cases of tricholemmal carcinoma with the ages of40-79y,including16cases whose scalp were involved.Positive staining of p53,PCNA and Bcl-2was found in72%,100%and63%of patients,re-spectively.The positive cells of p53were diffusely distributed.PCNA-positive cells were increased along with the degree of differentiation.Significant differences of Bcl-2expression were found among the various degrees of differentiation(P<0.01).Conclusions The lobulated construction and abrupt keratinization are hitologi-cal features of tricholemmal carcinoma.Different degrees of expression of p53,PCNA and Bcl-2might take part in neoplastic development and control.