结合珠蛋白在正常人皮肤中的定位表达

Preliminary Study of Haptoglobin Expression in Normal Human Skin

目的探讨正常人皮肤中结合珠蛋白及其mRNA的表达在皮肤中的作用及其与朗格汉斯细胞的关系。方法采用过氧化物酶法及荧光双标记法和原位杂交技术检测30例正常人皮肤中结合珠蛋白的表达。结果角质形成细胞、毛囊上皮、皮脂腺导管上皮细胞、腺上皮及小汗腺细胞结合珠蛋白mRNA呈弱阳性至中度阳性表达,真皮成纤维细胞和血管内皮细胞中均未见阳性表达,而在蛋白质水平,只见到一些树突细胞呈阳性表达,免疫荧光双标记检测证实为朗格汉斯细胞。结论皮肤是结合珠蛋白肝外表达的又一器官,角质形成细胞等上皮来源的细胞是结合珠蛋白mRNA表达的主要细胞;正常皮肤中只见到表皮中下部、毛囊、皮脂腺导管部位的朗格汉斯细胞胞浆中含有结合珠蛋白;角质形成细胞中结合珠蛋白mRNA的表达可能与朗格汉斯细胞胞浆中的结合珠蛋白有关;结合珠蛋白可能参与皮肤中的一些免疫反应过程。

Objective To investigate the expression of haptoglobin in normal human skin,its mRNA and protein levels,its role in the skin and the relationship with Langerhans cell.Methods The expression of haptoglobin in the skin was detected by immunohistochemistry and in situ hybridization in30normal individ-uals.Results Weak to mild expression of haptoglobin mRNA was found in keratinocytes,hair follicles,ec-crine glands,the ducts of sebaceous glands,and glandular epithelia.No expression was observed in dermal fi-broblasts and the endothelial cells of blood vessel.In immunohistochemistry study,positive staining was found in the cytoplasm of some dentritic cells.These dendritic cells were confirmed to be Langerhans cells by dou-ble-labelled immunofluorescence.Conclusions Skin is another extrahepatic organ where haptoglobin mRNA is expressed,mainly on keratinocytes and cells originated from epithelium.Expression of haptoglobin in the cytoplasm of Langerhans cells is observed only in the lower-middle epidermis,hair follicles,and the ducts of sebaceous gland.The expression of haptoglobin mRNA in keratinocytes might be related to the presence of haptoglobin in the cytoplasm of Langerhans cells.Haptoglobin might take part in the process of immune reac-tion in the skin.