实验性脑出血血肿周围血流量变化的研究

The study of cerebral blood flow after experimental intracerebral hemorrhage

目的 建立实验性脑出血的动物模型 ,探讨血肿周围的脑血流量变化。方法 采用犬脑内缓慢注入非肝素化自体血的方法建立实验性脑出血动物模型。用 1 4C- iodoantipyrine微示踪技术测定实验性脑出血 6 h、2 4 h、72 h血肿周围皮质、白质及对侧相应部位的脑血流量。结果 注血 6 h组、2 4 h组血肿周围白质的 CBF与对照组相比有所下降 ,但无统计学差异 ;而注血 72 h组血肿周围白质的 CBF较对照组降低了 19.8% (P<0 .0 5 ) ;注血 72 h组血肿对侧相应部位白质的 CBF较对照组升高了 14 .8% (P<0 .0 5 )。结论 犬脑内缓慢注入非肝素化自体血可建立可靠、重复性好的实验性 ICH动物模型 ,脑出血后 72 h,血肿周围白质的血流量下降 ,脑出血早期的脑损伤非缺血所致。

Objective To establish the reproducible model of experimental intracerebral hemorrhage(ICH),and measure the cerebral blood flow(CBF) around hematoma.Methods ICH was induced by autologous nonheparinized blood injection in dogs.The regional CBF(perihematomal white and gray matter and the corresponding regions in contralateral hemisphere) at 6h,24h and 72h was measured by indicator fractionation technique(using 14 C iodoantipyrine).Results The CBF decreased in the ipsilateral white matter at 6h and 24h afer ICH compared with that of the sham operated group, but it had no statistical difference. The CBF was reduced by 19.8% of the value in sham operated group in the ipsilateral white matter at 72h.The CBF increased 14.8% of the value in sham operated group in the contralateral white matter.Conclusion A controllable, reproducible model of experimental ICH can be developed by autologous nonheparinized blood injection;The CBF decreases in the ipsilateral white matter at 72h; Ischemia is not the cause for brain injury in earlier period of ICH.