摘要
目的 :研究干扰素 -γ(IFN γ)在胃癌中的表达规律及可能的意义。方法 :应用分子克隆方法 ,克隆IFN γ基因片断 ,并通过体外转录方法制备寡核苷酸探针。在石蜡包埋的 5 8例胃癌组织和 5 3例癌旁正常组织中 ,采用原位杂交方法检测IFN γmRNA的表达。结果 :癌旁组织中胃上皮细胞IFN γ阳性率为 4 9 1% ,而胃癌组织中未发现 1例阳性 ,两组间差异有极显著意义 ,χ2 =37 15 6 ,P =0 0 0 0。结论 :胃癌细胞不表达IFN γ ,可能与胃上皮细胞癌变及免疫逃逸有关。
Objective To study the expression of IFN γ in gastric cancer and its possible significance.Methods Fifty eight gastric paraffin wax embedded cancer tissues and fifty three normal tissues adjacent gastric cancer were tested for the expression of IFN γ mRNA by in situ hubridization,respectively.Results The positive rate of IFN γ in cancer cells of gastric cancer tissues was significantly lower than that in gastric epithelial cells of the tissues adjacent cancer(0 and 49 1%respectively,χ 2=37 156, P =0 000).Conclusions The expression of IFN γ is low in gastric cancer cells.It may be related to the carcinogenesis of gastric epithelial cells and might be responsible for the immune escape of these cells.
出处
《肿瘤防治杂志》
2003年第9期907-909,共3页
China Journal of Cancer Prevention and Treatment
基金
国家"8 63"项目资助 ( 2 0 0 2BA711A0 6)
关键词
胃肿瘤/免疫学
干扰素Ⅱ型/代谢
原位杂交
stomach neoplasms/immunology
interferon typeⅡ/metabolism
in situ hybridization
