双硫腙对母鼠锌代谢及仔代脑发育的影响

EFFECTS OF DITHIZONE ON Zn METABOLISM IN MATERNAL RATS AND THE DEVELOPMENT OF BRAIN OF THEIR PUPS

本实验给妊娠后期大鼠一定剂量的双硫腙(dithizone)灌胃1周,观察了母鼠粪锌、血清锌的改变,同时对其所产仔代大脑的发育状况进行了研究。结果表明:双硫腙连续灌胃给药1周后,母鼠24h粪锌排出量显著增加,血清锌水平显著下降。说明双硫腙可以造成实验性动物体內缺锌庵秩毙磕甘笏写奶逯亍⒛灾亍⒛缘鞍字逝ǘ纫约癛NA/DNA比值均显著下降。提示妊娠后期母鼠体內缺锌,将对仔代大脑发育产生不良影响。本文对上述结果进行了必要的讨论。

The changes of zine content in serum and feces observed after intragastric administration of a certain dose of dithizone to the pregnant rats in their third trimester for one week, and the developing status of brain of their pups were studied. The result showed that the total fecal exerction of zine during 24 hours increased and serum zine content decreased significantly aftcr continual administration of dithizone for one week in maternal rats. It suggested that dithizone may be used for making animal model of zine deficiency. The body and brain weights, brain protein content and RNA/DNA ratio of the newborn delivered by the zine-deficiency maternal rats decrcased significantly. This indicates that the maternal zine dificiency in third trimester gives side effects on the development of their fctal brain.