硒对大鼠心肌细胞膜Na^+,K^+—ATP酶及5'—核苷酸酶活性的影响

EFFECT OF Se ON Na^+ ,K^+-ATPase AND 5'-NUCLEOTIDASE ACTIVITIES OF CARDIAC SARCOLEMMA IN RATS

本文用克山病病区低硒粮、病区粮加Na_2SeO_3及常备饲料分别喂养Sprague—Dawley纯种大鼠1～3个月,动态观察了大鼠心肌细胞膜Na^+,K^+—ATP酶(Na^+,K^+—ATPase)及5'一核苷酸酶(5'—ND)活性变化。结果发现低硒组大鼠心肌细胞膜Na^+,K^+—ATPase和5'—ND活性从实验的第1到第3个月均明显低于补硒组和常备饲料组大鼠;低硒组大鼠肝脏和血浆硒含量及红细胞和肝脏的GSH—px活性也明显降低。补硒可使上述各项指标接近常备饲料组大鼠水平,表明硒对心肌细胞膜有稳定和保护作用。

Sprague—dawey rats were fed for one to three months with Se—deficient grains from Keshan disease area, Se—supplemented diet and stock diet,respectively. Changes of Na^+ ,K^+ ATPase and 5'—nucleotidase activities in cardiac sarcolemma ,Se Contents in liver and plasma and GSH—px activities in Liver and red blood cells of rats were observed. The results showed that Na^+ ,K^+—ATPase. and 5'—nucleotidase activities in cardiac sarcolemma in Se-deficient group rats were significantly decreased,hepatic and plasma Se contents as well as hepatic and erythrocyte GSH—px activities were also significantly decreased at of feeding 30,60 and 90 d as compared to Se—supplemented or stock diet group. There was no significant difference in these parameters between Se—supplemented or stock diet groups There was no significant difference in these paramefers between Se—supplemented and stock diet groups。