比值导数波谱法分析食品中抗氧化剂

DERIVATIVE RATIO SPECTRA-SPECTROPHOTOMETRIC DETERMINATION OF ANTIOXIDANTS IN FOOD

利用混合物的波谱除以干扰组分的波谱得比值波谱,对波长求导得比值导数波谱,由此可消除干扰组分的吸光度叠加效应。该法能有效地对三组分混合物体系进行分析。利用此法对没食子酸丙酯(PG)、2,4,5 三羟基丁酰苯(THBP)和叔丁基对苯二酚(TBHQ)所组成的三组分混合体系进行分析,结果满意。

The derivative ratio spectra is found by derivation of the ratio of the spectra of compounds in a mixture to the spectra of the interfering component against the wavelength. The interference of the addition effect of absorbances of interfering component is thus eliminated. This method is applied effectively to the analysis of three-componental mixture systems. For instance, three food-antioxidants (i.e. propy gallate (PG), 2,4,5-trihydroxybutyryl benzene (THBP) and tert-butylhydroquinone (TBHQ)) are determined simultaneously by this method satisfactorily.