对—氨基苯甲酸与亚硫酸氢钠对甲基硝基亚硝基胍的抗诱变作用

ANTIMUTAGENIC EFFECT OF P—AMINOBENZOIC ACID AND SODIUM EISULFITE ON THE MUTAGNICITY OF N-METHYL-N-NITRO-N-NITROSOGUANIDINE

本文采用人外周血淋巴细胞程序外DNA合成(UDS)方法检测对—氨基苯甲酸(PABA)、亚硫酸氢钠(NaHSO_3)对甲基硝基亚硝基胍的抗诱变作用,结果表明不同浓度的PABA,NaHSO_3对相同浓度的甲基硝基亚硝基胍(MNNG)的抗诱变作用,以PABA浓度为5×10^(-5)mol/L,NaHSO_3浓度为3×10^(-4)mol/L时,抗诱变作用最大。而PABA、NaHSO_3浓度恒定时,对不同浓度的MNNG的抗诱变作用,均以高浓度MNNG的抗诱变作用明显。因此认为PABA、NaHSO_3对MNNG具有抗诱变作用,其大小与MNNG的浓度有关。

Antimutagenic effect of p-aminobenzoic acid (PABA) and sodium bisulfite (NaHSO_3)on the mutagenicity of N-methyl-N-nitro-N-nitrosoguanidine (MNNG) was detected by the mehod of human lymphocytes unscheduled DNA Sythesis (LIDS). The results showed that when the concentration of PABA and NaHSO_3 was varied in presence of a constant concentration of MNNG, 5 ×10^(-5) mol/L concentration of PABA, 3 × 10^(-4) mol/L concentration of NaHSO_3 were the most effective, while the concentration of MNNG varied in the presence of a constant concentration of PABA and NaHSO_3, antimutagenicity was the most effective at high concentration of MNNG. The results indicated that the PABA and NaHSO_3 exhibited antimutagenic activity towards MNNG-induced mutagenicity in LIDS and the extents of antimutagenicity were related with the concentration of MNNG.