硒对大鼠心肌线粒体超氧阴离子自由基产生能力的影响

EFFECT OF SELENIUM ON GENERATION OF SUPEROXIDERADICAL IN RAT HEART MITOCHONDRIA

本文用低硒饲料(硒含量0.009mg/kg)和低硒饲料补亚硒酸钠(硒含量0.232mg/kg)分别喂养大鼠,对比观察喂养1,2和3个月时两组大鼠心肌线粒体超氧阴离子自由基(O_2)生成率、锰-超氧化物歧化酶(Mn-SOD)活性及血硒浓度。结果表明:低硒组大鼠心肌线抗体O_2生成率在喂养2和3个月时,分别显著高于同月补硒组;在喂养各月两组间Mn-SOD活性无显著性差别;低硒组大鼠血硒浓度各月均明显低于补硒组并逐月下降。提示硒可抑制心肌线粒体O_2产生能力。

Weanling rats were fed with a low-selenium diet (Se 0.009mg/kg, — Se group) and the diet supplemented with sodium selenite (Se 0. 232mg/kg,+ Se group) for 1, 2 and 3 months respectively. The generation rate of superoxide radical(O_2^-), activity of manganese dependent superoxide dismutase (Mn-SOD) in heart mitochondria and blood Se concentration from the rats were determined. The results showed that the mitochondrial O_2^- generation rate from — Se rats was significantly higher than those from + Se rats at 2 and 3 months. During all the feeding periods, no significant difference in mitochondrial Mn-SOD activity was found between the—Se and + Se groups,while the blood Se concentration in — Se group was significantly lower than those in + Se group at each feeding time and continually decreased in the 2 and 3 month. The results suggested that selenium could depress O_2^- generation in heart mitochondria.