青藤碱对吗啡依赖离体豚鼠回肠作用的实验研究

Effect of sinomenine on morphine dependence in isolated guinea pig ileum

目的探讨青藤碱对吗啡依赖性的戒断作用及机制。方法建立豚鼠离体回肠模型,以纳络酮催促吗啡依赖性戒断收缩,观察10、50和250 μmol/L Sin及0.1 μmol/L尼莫地平在加入纳络酮前后给药对戒断性收缩的影响;观察青藤碱对乙酰胆碱引起的正常豚鼠回肠收缩的作用。结果青藤碱及尼莫地平均可抑制纳络酮催促的戒断性收缩,对乙酰胆碱引起的正常豚鼠回肠收缩也有抑制作用,青藤碱的作用呈剂量依赖趋势。结论青藤碱能够抑制吗啡依赖的戒断症状。

Objective To investigate the effects of sinomenine on morphine withdrawal response and acetylchline (Ach)-in-duced contracture in isolated guinea pig ileum. Methods The withdrawal contracture was elicited by subjecting isolated ileum incubated with morphine (3 μmol/L) at 37.5 ℃for 4 h to naloxone (1 μmol/L) treatment. Sinomenine (10, 50, 250 μmol/L) and nimodipine (Nim, 0.1 μmol/L) were administered 1 min before and after naloxone in morphine-dependent ilea bathed in Krebs solution containing morphine, to observe the changes in the withdrawal contracture of the ileum. The effect of sinomenine (10, 50, 250 μmol/L) on the contracture of untreated ileum in Krebs solution elicited by acetylcholine was also observed. Results and Conclusion Naloxone-induced withdrawal contracture or acetylcholine-induced contracture of the ileum was significant-ly decreased in a dose-dependent manner, indicating that sinomenine can inhibit morphine withdrawal symptoms in guinea pigs.