胃癌组织中核基质蛋白与p16基因上游序列结合检测

Binding of the upstream of p16 gene to the nuclear matrix protein in gastric cancer

目的 :研究胃癌组织核基质蛋白与p1 6基因上游序列的结合情况及其意义。方法 :采用Southwestern印迹技术及Genetools定量分析软件 ,对 2 2例胃癌及相应正常组织的核基质蛋白与P1 6基因上游序列的结合进行了检测。结果 :正常胃组织核基质蛋白与p1 6基因上游序列结合主带的相对分子质量 (Mr)为 2 80 0 0、30 0 0 0、4 0 0 0 0、4 30 0 0、5 0 0 0 0 ,胃癌组织中为 4 0 0 0 0、6 6 0 0 0。与正常胃组织比较 ,胃癌组织中核基质蛋白与P1 6基因上游序列结合的6 0 0 0主带阳性信号明显增强 (P <0 .0 5 ) ;而不同分化程度及不同临床分期胃癌组织间 ,此主带阳性信号强度差异无统计学意义 (P >0 .0 5 )。结论 :胃癌组织中 6 6 0 0 0蛋白与p1 6基因上游区结合量的异常可能是胃癌发生的早期分子事件。

Aim: To study the binding of upstream of p16 gene to the nuclear matrix protein in gastric cancer. Methods: The Southwestern blot technique and Genetools quantitative analysis software were used to study the binding of nuclear matrix proteins to the upstream of p16 gene in 22 cases of gastric cancer and normal gastric tissues. Results: The binding bands of the upstream of p16 gene to the nuclear matrix protein were 28 000, 30 000, 40 000, 43 000, 50 000 in normal gastric tissues, versus 40 000, 66 000 in cancer tissues. The positive signal of 66 000 protein in cancer tissues was significantly higher than that in normal tissues ( P <0.05), but that had no significant difference between the various differentiated types and between the various clinical stages of gastric cancer tissues ( P >0.05). Conclusions: The alteration of the binding of 66 000 protein to the upstream of p16 gene in gastric cancer tissues might be an early molecular event in the development of gastric carcinoma.