食管脱落细胞DNA增殖活性的研究

The flow cytometric analysis of exfoliated cells in patients with esophageal carcinoma

目的通过流式细胞术测定食管脱落细胞DNA增殖活性指标,以探讨其在食管癌中的诊断价值。方法用食管拉网法获得41例食管癌病人和21例非食管癌病人食管脱落细胞,制成单细胞悬液,作碘化丙啶荧光染色,记录和计算组方图、DNA指数(DI)、G2G1、S期比率(SPF)、细胞增殖活性指数(PI)等指标,同时结合肿瘤的临床病理学特征,并与脱落细胞常规细胞学检查结果进行对比。结果DI:食管癌组1.31±0.23,对照组1.00±0.03;异倍体(An)出现率:食管癌组70.7%,对照组4.8%;G2G1:食管癌组2.071±0.153,对照组1.996±0.099;SPF:食管癌组28.830±15.997,对照组5.804±4.771;PI:食管癌组37.010±13.794,对照组14.738±9.703。两组上述指标的差别均有显著性意义(P<0.05或0.01)。结论食管癌组食管脱落细胞的DI、An以及PI均较对照组明显升高。食管脱落细胞DNA增殖活性指标对食管癌普查及提高早期诊断率具有一定的应用价值。

Objective To evaluate the clinical significance of flow cytometry analysis in early diagnosis of esophageal carcinoma.Methods The exfoliated cells were obtained by the esophagus fleece-pulling from 41 patients with esophageal carcinoma and 21 non-cancer patients.The single-cell suspension was made by mechanical decentralization and examinated by flow cytometry for DNA index(DI),G2/G1,S-phase fraction ( SPF ) and proliferation index ( PI ).The flow cytometric results were compared with those of routine cytology examination.Results The DI of esophageal carcinoma group and the control group was 1.31±0.23 and 1.00±0.03 (P<0.01)respectively;the aneuploid rate of in esophageal carcinoma group was 70.7%,while that of control group was 4.8% (P<0.01);the G2/G1 ratio for esophageal carcinoma group was 2.071±0.153 and that for control group was 1.996±0.099 (P<0.05);the SPF of esophageal carcinoma group was 28.830±15.997, that of control group was 5.804±4.771(P<0.01);the PI of esophageal carcinoma group was 37.010±13.794 and that of control group 14.738±9.703 (P<0.01).Conclusion DNA analysis of exfoliated esophageal cells by flow cytometry may have significant value for early diagnosis of esophageal carcinoma.