小鼠囊胚与不同侵袭转移潜能肝癌细胞系共培养模型的建立及生物学行为观察

Model establishment and biological behaviour observation of mouse bla stocyst co-cultured with hepatocarcinoma cell lines with differently invasive and metasta tic potential in vitro

目的 :探讨两种生命形式细胞 -囊胚细胞和肝癌细胞在体外相同微环境下的相互作用及整体生物学行为变化。方法 :建立小鼠囊胚与人肝癌细胞共培养模型 ,观察两种生命形式细胞在共培养系统中的生物学行为及相互影响。结果 :与对照组相比 ,不同侵袭转移潜能的肝癌细胞显著提高囊胚的脱带率、贴附率和扩展率 (P <0 0 5) ,但肝癌细胞各组之间无显著差异 (P >0 0 5)。囊胚细胞能在不同侵袭转移潜能的肝癌细胞上进行脱带、粘附 ,分化的滋养层细胞能侵袭肝癌细胞并在两者之间形成清楚的边界。界面上的肝癌细胞有生长方向和细胞形状的改变且不能侵袭囊胚细胞。结论 :肝癌细胞能促进囊胚的发育。囊胚细胞能植入和侵袭肝癌细胞 ,表明囊胚细胞在体外植入与相作用细胞的种属和分化水平无关 。

AIM: To explore interaction and biological behaviou r changes of two kinds of cells-blastocysts and hepatocarcinoma cells in the same microenvi ronment. METHODS:The models of mouse blastocysts co-cultured wit h human hepatoca rcinoma cell lines were established, then biological behaviours and mutual effe c ts of the two kinds of cells in co-culture system were observed. RESULTS: Co mpared with control group, hepatocarcinoma cells with differently invasive and met astatic potential significantly enhanced the rates of blastocyst hatchment , at t achment and outgrowth(P<0.05). There was no significant difference in tho se among hepatocarcinoma cells co-cultured groups (P>0 05). The blastocyst ha tched and attached to hepatocarcinoma cells with differently invasive and metast atic p otential. Then, differential trophoblasts invaded hepatocarcinoma cells. The clear-cut interfaces were gradually formed between both sides. Hepatocarcinoma cells o n interface showed changes of growth direction and cell shapes and did not inv ade blastocysts. CONCLUSIONS: Hepatocarcinoma cells promoted bla stocyst develo pment. Blastocysts implanted and invaded hepatocarcinoma cells with differentl y i nvasive and metastatic potential in vitro, which indicate that blastocyst i mplan tation in vitro does not relate with the kinds and differential level of int erac tional cells and the low selectivity maybe relate with high adaptability of earl y life.