小剂量MIP-1α联合IL-8体外对脐血CD34^+细胞集落形成的影响

The effect of low dose MIP-1α combined IL-8 on cord blood CD34^+ cells′ clone formation

目的 了解巨噬细胞炎症蛋白 1 α(macrophageinflammatoryprotein 1α,MIP 1α)联合白细胞介素 8(interleukin 8,IL 8)体外对脐血造血细胞集落形成的影响。方法 采用免疫磁珠吸附法分离脐血CD34+ 细胞 ,根据随机单位组方差分析设计 (ran domcompleteblockdesignvarianceanalysis) ,将每例标本分为空白组 (不加MIP 1α和IL 8) ;1 0ng/ml联合组 (加入 1 0ng/ml的MIP 1α和IL 8) ;1ng/ml联合组 (加入 1ng/ml的MIP 1α和IL 8) ;单用MIP 1α组 (加入 2 0ng/ml的MIP 1α) ;单用IL 8组 (加入 2 0ng/ml的IL 8) ,建立半固体培养体系 ,一定时间后 ,观察半固体体系中的集落生长情况。结果 集落培养表明 1 0ng/ml的浓度下 ,两者联合使用 ,在体外能明显地抑制脐血造血细胞各种集落的形成 ,其它组均未见明显的集落形成抑制作用。结论 1 0ng/mlMIP 1α与IL

Objective To study the effect of low dose MIP 1α combined IL 8 on cord blood cells' clone formation.Method Cord blood MNC were isolated from other cells by centrifugation in medium of Ficoll Hypagque (1.007).After that,CD34 + cells were got from these MNC buy MiniMACS,and then these CD34 + cells were divided into five groups : control group,10ng/ml MIP 1α combined 10ng/ml IL 8 group,1ng/ml MIP 1α combined 1ng/ml IL 8 group,20ng/ml MIP 1αgroup and 20ng/ml IL 8 group.Then these cells were cultivated in 0.9% methylcellulose system.Several days later,count the clones of CFU GM,CFU E and CFU Mix of each group.Result At the concentration of 1ng/ml MIP 1α combined IL 8 can not inhibit the clone formation obviously,but 10ng/ml MIP 1α combined IL 8 in same concentration can inhibit the clone formation obviously.Neither MIP 1α nor IL 8 can inhibit the clone formation if they were used alone in our study.Conclusion 10ng/ml MIP 1α combined IL 8 can synergies each other to inhibit cord blood cells,clone formation.