中国部分乌鸡品种血清酶蛋白多态性研究

STUDIES ON THE SERUM ENZYME PROTEIN POLYMORPHISMS IN PART OF CHINESE BLACK BONE CHICKENS

采用聚丙烯酰胺凝胶电泳法测定鸡血清脂酶(E_(?)-1),碱性磷酸酶(Akp)、碱性磷酸酶-2(Akp-2)的多态性结果表明,E_(?)-1由常染色体上等显性等位基因E_(?)-1~A,E_s-1~B所支配,其中E_(?)-1~B基因在我国药用和肉用乌鸡中出现频率较高(0.7768～0.8700),而E_s-1~A基因在蛋用型莱航和罗斯鸡中占优势(0.6169～0.6818)。Akp、Akp-2均由显隐性基因所控制,其出现频率亦因鸡种不同而表现一定的差异。试验中发现有些个体E_s-1~A基因可进一步区分为E_s-1^(A1)和E_s-1^(A2),从而使所研究鸡种中E_s-1的等位基因数目扩展至3个。

Serum samples of chickens were analysed for their esterase-1 (E_S-1), alkaline phosphatase (Akp) and alkaline phosphatase-2 (Akp-2) polymorphisms with polyacrylamide gel electrophoresis. The results showed that E_S-1 was controlled by the codominant autosomal alleles of E_S-1~A and E_S-1~B and the latter had the higher frequencies in Chinese medicine and meat black bone chickens (0.7768～0.8700),but the former had the dominant freauencies in laying chickens of white Leghorn and Ross Brown (0.6169～0.6818). Akp,Akp-2 were both controlled by the dominant and resessive genes at an autosomal locus and the frequencies of them had some differences because of different breeds. It was discovered that some of the E_S-1~A gene could be subdivided into E_S-1^(A_1) and E_S-1^(A_2) and therefore the E_S-1 alleles in the chickens studied were expanded to 3 ones.