K型小麦雄性不育体系育性恢复的遗传分析

GENETIC ANAYSIS OF MALE-FERTILITY RESTORATION IN WHEAT WITH AE. KOTSCHYI CYTOPLASM

选用两个K型小麦雄性不育系K7859A和K80(6)A分别与6个恢复系杂交,F_1自交并与母本不育系回交得到12个F_2和回交一代群体。分离群体的育性反应可划分成完全可育、中度可育、低度可育和完全不育4级,比例分别为10:3:2:1和1:1:1:1。结果表明,K型小麦雄性不育体系的育性恢复受两对非等效基因控制,恢复作用较强的基因定为Rfk_1,较弱者定为Rfk_2。它们的显性作用都不完全,具有累加效应,基因型为Rfk_1rfk_1Rfk_2rfk_2时即表现完全可育。

Two male-sterile lines of wheat (with Ae. kotschyi Cytoplasm), K7859A and K80(6)A, were crossed with six R-lines, and twelve F_2 and BC_1F_1 generations were obtained by F_1 plants selfing and from the crosses of F_1 as male with the maternal parent respectively. The ratio of complete fertility: middle fertility: low fertility: sterility in F_2 was 10: 3: 2: 1, and in BC_1F_1 was 1: 1: 1: 1. The results indicate that the fertility restoration involves two major genes. The restoring effect of these genes is unequal, incompletely dominant, and acts in a additive manner. The stronger restorer-gene was symbolized as Rfk_1 and the weaker one as Rfk_2. The plant with genotype of Rfk_1rfk_1Rfk_2rfk_2 is complete male fertility.