迎春花中黄酮类化合物含量的研究(英文)

STUDIES ON THE CONTENT OF FLAVONOIDS IN JASMINUM NUDIFLORUM LINDL.

以 75 %乙醇为溶剂 ,索氏提取器提取 ,采用比色法和HPLC法研究了迎春花中黄酮类化合物的含量。以芦丁为对照物 ,比色法测定出其花、叶、茎中总黄酮的含量分别为 13.7%、16 .2 %、6 .77% ,RSD分别为 2 .6 3%、2 .5 7%、2 .96 % ;以槲皮素、山奈素、异鼠李素为对照物 ,HPLC法测定出其花、叶、茎中的黄酮总量 ,以甙元计分别为 4 .2 0 5 %、3.371%、1.70 3% ,RSD分别为 1.76 %、1.5 8%、1.98%。

The flavonoids in Jasminum nudiflorum Lindl. extracted by Soxhlet extractor with 75% ethanol was studied by colorimetry and HPLC. Determined by colorimetry with rutin as standard sample, the content of total flavonoids in its flowers, leaves and stems were 13.7%,16.2% and 6.77%,RSD were 2.63%, 2.57% and 2.96% respectively, while determined by HPLC with quercetin, kaempferol, isorhamnetin as standard samples, the content of total flavonoids in its flowers, leaves and stems counted by aglycone were 4.205%, 3.371%, 1.703%, RSD were 1.76%, 1.58% and 1.98% respectively.