摘要
目的探讨外周血CEA mRNA及癌组织中CD44v6的表达对胃肠道癌转移的影响及临床意义。方法采用RT-PCR技术和S-P免疫组化法进行研究。结果62例胃、结直肠癌患者术前外周血CEA mRNA RT-PCR检测阳性表达率为43.5％(27/62),对照组测定均为阴性。癌组织中CD44v6阳性表达率为46.7％(29/62)。Ⅰ+Ⅱ与Ⅲ+Ⅳ期胃肠道癌患者之间CEA mRNA、CD44v6的阳性表达差异均有显著性(P<0.05)。CD44v6、CEA mRNA的表达与局部淋巴结转移密切相关(P<0.05,P<0.01)。外周血CEA mRNA的阳性表达与癌组织中CD44v6的表达密切相关(P<0.01)。结论CEAmRNA是判定胃肠道癌微转移的敏感指标,与癌组织中CD44v6的表达密切相关,均可作为胃肠道癌的预后判断指标,对临床正确分期及综合治疗起指导作用。
Objective To study the relationship between the expression of CEA mRNA in peripheral blood, CD44v6 in cancer tissue and metastasis of gastrointestinal carcinomas. Methods Reverse transcription-poly-merase chain reaction (RT-PCR) and S-P immunohistochemical technique were used to detect CEA mRNA and CD44v6 respectively. Results The CEA mRNA was detected in 27 cases with gastrointestinal carcinomas before operation,the positive rate is 43.5%(27/62) ,but not found in control group.The CD44v6 was found in 29 cases, with positive rate of 46.7 % (29/62). There was a high correlation between the expression of CD44v6 and CEA mR-NA( P < 0.01), also between the expression of CD44v6 and CEA mRNA and metastasis of regional lymph nodes (P<0.05,P<0.01). The significant difference in the expressions of CEA mRNA and CD44v6 was noted between stages Ⅰ + Ⅱ and Ⅲ + Ⅳ(P < 0.05) .Conclusions The expression of CEA mRNA in peripheral blood is a sensitive indicator for early detection of micrometastasis. In addition, the level of expression of CD44v6 might correlate with metastasis by blood spread in gastrointestinal carcinoma. Both of them can be utilized as indexes of prognosis and as the guide for staging and treatment.
出处
《中国肿瘤临床与康复》
2003年第6期512-515,共4页
Chinese Journal of Clinical Oncology and Rehabilitation
