微量DNA的短串联重复序列分型可行性

Genotype reliability of short tandem repeats typing from minute DNA

目的了解微量DNA分型的法医学应用的可行性。方法一系列浓度的DNA模板用PowerPlexTM16System试剂盒扩增,并用ABI377DNA测序仪进行短串联重复序列(STR)的分型。结果当模板量小于250pg时,部分位点发生了等位基因漏扩,并出现非特异带、杂合子扩增不平衡等干扰分型的杂峰。结论上述这些不正常的现象可能会导致分型错误。在对微量检材的DNA检验结果进行判型时一定要小心谨慎,全面考虑。

Objective To understand the forensic practice of DNA profiling from minimal amount of DNA.Methods Serial dilutions of DNA were amplified with the PowerPlex TM 16System Kit,then the genotyping of short tandem repeat(STR)was performed by ABI377DNA automated Sequencer.Results When the mount of DNA template was less than250pg,allelic drop-out apparently occurred at several loci.Other disturbed peaks,such as artefact bands and imbalanced heterozygote,also presented.Conclusion The anomalous results may result in incorrect genotyping.Careful and comprehensive considerations are needed to interpret the STR profile of minute DNA.