脐血单个核细胞的抗肿瘤细胞活性

Antitumor Activity of Cord Blood Mononuclear Cells

目的 :探讨脐血单个核细胞对HO9810肿瘤细胞株的杀伤活性 ,并观察重组人干扰素α 2b(rhIFNα 2b)的调节作用。方法 :采集脐血及成人周围血 ,将分离所得的单个核细胞按是否添加处理因素分组进行培养 ,2 4h后用四甲基偶氮唑蓝 (MTT)法检测对HO9810细胞的杀伤作用。结果 :静止脐血单个核细胞对HO9810肿瘤细胞的杀伤活性较弱 ,经rhIFNα 2b激活后显著增强 ,P <0 0 5 ,与成人外周血单个核细胞的杀伤活性差异无显著意义 ,P >0 0 5。结论 :脐血单个核细胞能被rhIFNα 2b激活为杀伤细胞 ,激活后对HO9810细胞株有较强的杀伤作用 。

Objective To explore the killing efficacy of cord blood mononuclear cells in HO9810 and the activation effect of rhIFNα-2b on it.Methods Mononuclear cells were isolated from fetal blood and adult peripheral blood respectively,whose killing efficacy in HO9810 at the presence or absence of rhIFNα-2b were measured with MTT.Results The natural killing efficacy of cord blood mononuclear cells was lower than that of the peripheral blood.When activated,however,it was enhanced markedly and could be equal to that of the latter.Conclusions The mononuclear cells in the cord blood can be activated into killing cells by rhIFNα-2b,which shows increased killing efficacy to HO9810 and have a promised future in antitumor immunotherapy.