白细胞介素-18转导人大肠癌细胞株的建立及其生物学活性的研究

Study on Characterization Tumorgenesis of Interleukin-18 Transduced Human Colon Adenocarcinoma Cells

目的 :建立转白细胞介素 18(IL 18)基因人大肠癌细胞株 ,并研究IL 18的表达情况。方法 :将携带人白细胞介素 18的质粒pcDNA3 1 hIL 18转染到人大肠癌细胞系SW 4 80中 ,通过药物GENETI CIN(G 4 18Sulfate)进行筛选 ,利用PCR、RT PCR及ELSIA法对IL 18的表达进行检测。结果 :IL 18基因成功的转导入SW 4 80细胞中 ,10 6个转染细胞在 2 4h内分泌IL 18的含量是 112 9± 17 6pg ;空载体转染的细胞和未转染的SW 4 80细胞不产生IL 18。结论 :人IL 18基因成功的整合到大肠癌细胞系SW 4 80细胞基因组中 。

Objective To study the feasibility of interleukin-18 gene transfer to human colon carcinoma cell line SW480.Methods human colon carcinoma cell line SW480 was transfected with Lipofectamine 2000 and selected by G418,the expression of rhIL-18 was tested by PCR?RT-PCR and ELISA methods.Results The hIL-18 cDNA was successfully integrated into SW480 cell,it was (112.9±17.6) pg hIL-18 secreted by one million transduced cells in 24 hours.Conclusion The IL-18 gene was successfully integrated into SW480 cell line and could be expressed.