一种快速提取分析微囊藻毒素的方法

A RAPID EXTRACTING AND ANALYZING METHOD FOR MICROCYSTIN

以五种群体和单细胞微囊藻及自然微囊藻水华为材料 ,在沸水浴中经不同时间处理 ,过滤后直接进行HPLC UV检测 ,发现 12min的沸水浴处理就足以达到抽提目的。研究中发现 ,去离子水比蒸馏水是更有效的抽提溶剂。传统的甲醇抽提结果与沸水浴处理的相对误差主要在 0 2 %— 16 5 9%之间。结果还显示 ,群体微囊藻需要比单细胞微囊藻抽提更长时间。本研究提供了一种经过改进的高效、廉价和快速的微囊藻毒素抽提分析方法。

Microcystins are a group of natural toxins known to be produced by certain types of freshwater blue-green algae and which have been shown to be liver toxins and tumour promoters, and pose a health hazard for humans, domestic animals and wildlife. The traditional methods for toxin extraction include 5% acetic acid, 75% methanol, 100% methanol, 70% acetonitrile and so on. The extracting solution using traditional methods should be firstly pass through a sep-pak cartridge, then was rinsed with water and 25% methanol in water, and then microcystins were eluted with 90% methanol water, which is very overelaborate and expensive. In this paper, a new rapid method for toxin extraction was provided based on boiling-water bath, and this method were used to extract cultures of five strains of colonial or single-celled Microcystis and one natural water bloom sample. With different times dealing in boiling-water bath, and through 0.45μm membrane, it was detected directly by HPLC-UV, and it was found that twelve minutes boiling-water bath was enough for microcystin extraction. Comparing with the traditional extraction methods, the comparative error of boiling-water bath extraction method was from 0.2% to 16.59%. The results illustrated that more time was needed for extracting toxins from colonial Microcystis than from single-celled one. This study provides an efficient, cheap and quick extracting method for microcystin, which reveals a comprehensive and applied prospect.