胶质瘤对亲细胞非均质分子脂质和几种药物敏感性的比较

Contrast of the Sensitivity of Cytotropic Heterogeneous Molecular Lipids and Conventional Chemotherapy Drugs in Gliocytoma Cell

目的 应用药物敏感试验 (MTT比色法 )比较亲细胞非均质分子脂质 (CHML)和几种常用化疗药对脑胶质瘤细胞的抑制作用。方法 取 90例手术切除的脑胶质瘤新鲜标本 ,处理后接种于 96孔培养板中 ,加入不同浓度的CHML、VM 2 6、VP 16、CBP、DDP和福莫司汀 (fotemustine) ,孵育后进行MTT反应 ,于波长 5 70nm处测定A值。结果 胶质瘤细胞对CHML 5 0 0 μg/mL组的敏感率 (6 3/ 90 )、2 0 0 μg/mL组的敏感率 (5 8/ 90 ) ,显著高于 10PPC(血浆峰浓度 )VP 16、CBP、福莫司汀组 ,与 1PPCVM 2 6、10PPCDDP敏感率相似。 10 0 μg/mL浓度组CHML的敏感率要高于 0 .1PPC组的所有 5种药物。在耐药率方面 ,5 0 0、2 0 0 μg/mL浓度组的CHML耐药率只高于 10PPCVM 2 6 ,低于或相近于其余任何实验浓度药物。 10 0 μg/mL组的CHML耐药率低于 0 .1PPC组的所有 5种药物。 5 0 0与 2 0 0 μg/mL组CHML的敏感率和耐药率差异无显著意义 ,两者与 10 0 μg/mL组差异有显著意义。

Purpose: To contrast the inhibition of cytotropic heterogeneous molecular lipids(CHML) and conventional chemotherapy drugs in gliocytoma by drug sensitivity testing(MTT assay). Methods: Ninety tumor specimens were obtained and planted into 96 well plates immediately after operation. Incubated with CHML, VM-26, VP-16, CBP and DDP at different concentrations, MTT assay were taken. A values were detected at the wave of 570 nm length. Results: The sensitive rate of CHML at 500 μg/mL(63/90) and 200 μg/mL(58/90) were similar to 1 PPC VM-26 and 10 PPC DDP and higher than VP-16, CBP, fotemustine at 10 PPC. The sensitive rate of CHML at 100 μg/mL was higher than any other chemotherapy drugs at 0.1 PPC. The resistant rate of gliocytoma to CHML at 500 and 200 μg/mL were only higher than 10 PPC VM-26 but similar or lower than other chemotherapy drugs at different densities. The resistant rate of CHML at 100 μg/mL was lower than any other five drugs at 0.1 PPC. The sensitive and resistant rates of CHML at 500 and 200 μg/mL have no statistical differences, but significantly higher than that at 100 μg/mL. Conclusions: CHML is a effective agent to inhibitate vitro-incubated primitive gliocytoma.