大鼠腰髓背角星形胶质细胞及神经元对一侧胫、腓骨骨折的反应及其相互关系

RESPONSE AND RELATIONSHIP OF THE SPINAL CORD ASTROCYTES AND NEURONS TO PAIN INDUCED BY UNILATERAL TIBIA AND FIBULA FRACTURE IN RAT

目的 研究大鼠脊髓内星形胶质细胞及神经元对一侧胫、腓骨骨折的反应及相互关系。 方法 应用细胞免疫组织化学方法 ,观察脊髓内胶质原纤维酸性蛋白 (GFAP)、Fos蛋白双标记以及蛋白激酶C(PKC)在左侧胫、腓骨骨折后不同时程的表达变化。 结果 1 左侧胫、腓骨骨折后 ,GFAP阳性反应产物主要分布于同侧腰髓背角的星形胶质细胞胞浆 ,Fos阳性产物在其胞核也有表达 ,且以浅层为主 ,神经元的胞核也有Fos阳性产物 ;2 Fos LI神经元与GFAP Fos LI星形胶质细胞的分布基本一致 ,两者关系密切 ;3 GFAP Fos -LI星形胶质细胞表达高峰期在骨折后 4 5min ,而Fos LI神经元的表达高峰期在骨折后 90min ;PKC LI星形胶质细胞出现及达到高峰的时间比PKC LI神经元要早。 结论 腰髓背角的星形胶质细胞参与了下肢骨折伤害性刺激的应激反应及调节过程 ,而且Fos LI、PKC LI的表达早于神经元 ,可能主动地影响神经元的活动。

Objective To observe the instant reactions and relationship of astrocytes(AS) and neurons in rat spinal cord after the unilateral tibia and fibula bone fracture. Methods With single or double immunohistochemical staining method,the expression of Fos-like immunoreaction(-LI), glial fibrillary acidic protein(GFAP-LI), and protein kinase C(PKC-LI) were observed. Results Here we showed 1^ After the nociception of the treatment the PKC-LI,Fos-LI astrocytes and neurons,and GFAP-LI astrocytes were distributed in dorsal horn of rat lumbar spinal cord,predominantly in the superficial layer.2^ The distribution of Fos-LI neurons and Fos/GFAP-LI AS in dorsal horn of rat lumbar spinal cord were similar,there were close relationship between Fos-LI neurons and GFAP/Fos-LI AS.3^ The peak time of GFAP/Fos-LI AS expressive was 45?min after fracture,while that of Fos-LI neurons was 90?min,the peak time of PKC-LI astrocytes expressive was earlier than that of PKC-LI neurons.Conclusion The AS as well as neurons in dorsal horn of rat lumbar spinal cord might be involved in the instant response and adjustment of the lower extremity bone fracture nociception simultanneously.