摘要
目的 评价人胚胎干细胞建系与囊胚质量、原代克隆生长的关系。 方法 D3废弃胚胎成组共培养获得不同质量的囊胚 ,免疫刀去除囊胚滋养外胚层细胞后 ,将内细胞团 (ICM)接种到饲养细胞层上生长、传代。 结果 从质量好的囊胚得到的人胚胎干细胞传代的代数更多 ;原代克隆生长快的人胚胎干细胞传代效率更高。 结论 人胚胎干细胞建系与囊胚质量、原代克隆生长情况密切相关。
Objective Try to determine the relationship between blastocyst quality,the clonal growth of inner cell mass(ICM)and the establishment of human embryonic cell line. Methods Coculture D 3 discarded embryos with mouse embryonic fibroblast cells(MEFs).Then remove trophoectoderm by immunosurgery after getting different quality blastocysts.Culture ICM and passage these cells on MEFs. Results Human embryonic stem cells derived from good quality blastocysts could be passaged further than that from poor quality blastocysts,and ICMs growing fast could be passaged more quickly and efficiently.Conclusion The establishment of human embryonic stem cells is closely related with blastocyst quality and the original growth of ICM.
出处
《解剖学报》
CAS
CSCD
北大核心
2003年第6期652-656,共5页
Acta Anatomica Sinica
基金
广东省自然科学基金资助项目 (0 83 0 17)
