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大鼠三叉神经尾侧亚核内星形胶质细胞对疼痛刺激的反应及与神经元的关系 被引量：3

THE RESPONSE AND RELATIONSHIP OF RAT SPINAL TRIGEMINAL NUCLEUS ASTROCYTES AND NEURONS TO PAIN INDUCED BY SUBCUTANEOUS FORMALIN INJECTION
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摘要 目的　研究大鼠三叉神经尾侧亚核 (Sp5C)星形胶质细胞对唇下注射福尔马林所致疼痛的反应及其与神经元的关系。　方法　用免疫组织化学方法 ,显示注射后不同时间Sp5C星形胶质细胞与神经元内抗磷脂酶C(PLC)、抗Fos和抗胶质原纤维酸性蛋白 (GFAP)免疫组织化学产物的表达和分布。　结果　正常大鼠Sp5C无免疫组织化学阳性染色 ,唇下注射福尔马林后 ,Sp5C内的星形胶质细胞出现抗PLC、抗Fos和抗GFAP阳性染色 ,神经元出现抗PLC和抗Fos阳性染色 ,且有相同的亚核分布 ,关系密切。抗PLC和抗Fos免疫组织化学阳性先出现于星形胶质细胞 ,而后在神经元出现表达。　结论　Sp5C内星形胶质细胞可能参与中枢神经系统对疼痛刺激的调节 ,并主动调节神经元的活动。 Objective To investigate the responses of neurons and astrocytes within rat′s spinal trigeminal nucleus(Sp5C) following nocuous pain stimulation induced by subcutaneous formalin injection into the unilateral upper lip. Methods By means of anti\|phospholipase(PLC),anti\|Fos protein and anti\|glial fibrillary acidic protein(GFAP) immunohistochemical method,the distribution of reactive neurons and astrocytes in the Sp5C was observed at different time after formalin injection. Results No immunohistochemical staining was found in normal rat Sp5C.After formalin injection,PLC like immunoreaction(\|LI),Fos\|LI and GFAP\|LI were found in astrocytes while PLC\|LI and Fos\|LI in neurons.The labeled astrocytes and neurons showed similar distribution and close relationship.PLC\|LI and Fos\|LI were found earlier than that in neurons.Conclusion\ The results suggested that the astrocytes in the Sp5C might participate in pain modulation in CNS and actively regulate neuron′s function.
作者 袁华 段丽 饶志仁
机构地区 第四军医大学全军神经科学研究所
出处 《解剖学报》 CAS CSCD 北大核心 2003年第6期563-567,共5页 Acta Anatomica Sinica
基金 军队"十五"重点项目基金资助 (0 1Z0 82 )
关键词 三叉神经尾侧亚核 星形胶质细胞 疼痛 抗磷脂酶 免疫组织化学 福尔马林 Pain Spinal trigeminal nucleus Astrocytes Neurons Immunohistochemistry Rat
分类号 R322.81 [医药卫生—人体解剖和组织胚胎学]
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同被引文献26

  • 1兰莉,高蓓,饶志仁.脂多糖对大鼠中脑和桥脑内Fos、GFAP、OX42表达的影响[J].解剖学报,2005,36(1):24-27. 被引量：3
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解剖学报
2003年 第6期

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