涎腺腺样囊性癌中P_(16)蛋白表达与病理分型及复发的关系

Correlation Between P_(16) Protein Expressin and Pathological Types and Recurrence of Human Salivary Adenoid Cystic Carcinoma

目的 :探讨P16 蛋白在涎腺腺样囊性癌中表达与病理分型及复发的关系 ,为临床提供治疗及判断预后资料。方法 :利用免疫组化SP法 ,对 36例涎腺腺样囊性癌组织P16 蛋白表达情况进行检测 ,并对患者进行 10年随访观察。结果 :36例腺样囊性癌中 ,P16 蛋白阳性表达 12例 ( 33 3% ) ;实性型中P16 蛋白阳性率 ( 0 )明显低于筛孔型和腺管型P16 蛋白阳性率( 43 35% ) ,差异有统计学意义 ( P=0 0 33) ;有神经侵犯癌组织中P16 蛋白阳性率为 4 5 4 5% ,无神经侵犯P16 癌组织中蛋白阳性率为 2 8% ,两者间差异无统计学意义 (P =0 4 46 ) ;复发癌组织中P16 蛋白阳性率 ( 8 33% )低于无复发癌组织中P16蛋白阳性率 ( 45 83% ) ,两者间差异有统计学意义 (P =0 0 31)。结论 :P16 基因突变、缺失与腺样囊性癌病理类型有关 ,其表达水平反映肿瘤组织分化程度和恶性度 。

Objective: To study the relationship between the P 16 pr ote in expression and pathological types, recurrence and nerve invasion of human salivary adenoid cystic carcinoma, and t o provide clinical data for tumor treatment and prognosis judgement. Methods: By immunohistochemical SP staining method, P 16 protein expression was detected in 36 cases of ACC. The patien t s were followed-up as long as 10 years. Results: The P 16 pro te in expression rate in adenoid cystic carcinoma tissue were 33.33%. The P 1 6 protein expression rate in solid type was significantly lower than that in c ribriform and tubular types(P<0.01,P<0.05). No correlation was found b etween P 16 protein expression in the carcinoma with and without nerve invasion ( P>0.05 ). The P 16 protein expression rate in recurrance carcimoma was lower than that in primary carcinoma (P<0.05). Conclusion: The mutation and deletion of P 16 gene and corresponding alteration of P 16 protein ex p ression were related to pathological types,grades and differentiation degree of carcino ma. The detection of P 16 protein could be used to evaluate adenoid cystic carcinoma malignant degree and patient's prognosis.