一种新型生化需氧量传感器的研制

Development of a new biosensor for biochemical oxygen demand

目的 研制快速测定生化需氧量 (BOD)的方法。方法 应用溶胶 凝胶有机 无机杂化材料 ,采用包埋法固定异常汉逊酵母菌 ,制备微生物膜。该微生物膜与氧电极结合制成传感器。传感器测试的最佳条件为 :pH7 0 ;2 8℃ ;响应时间 3～ 12min。用该传感器测定了单纯有机物、生活污水及回收率。结果 经过对多种有机物测定表明该BOD微生物传感器可监测氨基酸类、糖类等多种有机物 ,适合于生活污水及某些含醇类、酚类等有机物浓度小的工业废水的测定。微生物膜可连续保存 3个月仍能保存其活性的 70 %。经测定生活污水的回收率在 90 %～ 10 5 %之间。结论 这种微生物传感器可成功进行BOD的测定 ,检测速度快 ,操作与制备简便 ,灵敏度高 ,适用范围广 ,成本低 ,易保存 ,性能稳定 ,简化了传统的测定分析 ,具有很好的实用意义。

Objective To use a new kind of fixing material,i.e.Sol-Gel organic-inorganic hybridized material to immobilize bacterium to detect Biochemical oxygen demand quickly. Methods The biosensor was fabricated using a thin film in which Hansenula anomala was immobilized by sol-gel and an oxygen electrode.The optimum measurement for biochemical oxygen demand was at pH 7.0; 28℃; response time 3-12 min.Pure organic compound, sewage and rate of recovery were detected with the biosensor. Results It shows that the BOD biosensor can be used to detect many organic compounds such as amino acid, glucide.It is suitable to monitor sewage and industrial waste water which has low level alcohols and phenols.The microbial membrane can work 3 months and remain its 70% activity.It is measured that the rate of recovery of BOD is between 90% to 105% in sewage. Conclusion The study confirmed the effectiveness and usefulness of BOD sensor, which is quick, convenient, low cost and reliable with little interference.