腺病毒携带反义HSP70抑制人喉癌细胞的生长

Anti-growth of laryngeal cancer by adenoviral mediated antisense HSP70

目的 :观察HSP70反义RNA对人喉癌细胞Hep 2的作用 ,探讨其治疗喉癌的可行性 .方法 :应用电镜、流式细胞仪和免疫组化图像分析技术检测将携带反义HSP70腺病毒感染人喉癌细胞后 ,诱导细胞凋亡和HSP70基因表达情况 .结果 :HSP70反义RNA阻断了Hep 2细胞的HSP70的表达 ,实验组可以见到典型的凋亡细胞 ,免疫组化证实 ,实验组瘤细胞低表达HSP70 ,而对照和空载体组高表达HSP70 .转染反义HSP70的腺病毒的Hep 2细胞比空载体组和对照组的细胞生长缓慢 ,细胞周期可见实验组出现亚二倍凋亡峰 ,而空载体组没有 .结论 :HSP70反义RNA能显著减少人喉癌细胞内HSP70的表达 ,具有抑制肿瘤生长的作用 .

AIM: To observe the role of HSP70 antisense RNA in the human laryngeal cancer cell line and to explore the therapeutic potentials in treating laryngeal cancer. METHODS: By means of transmissional electron microscope, flow cytometer (FCM) and images analysis, we observed apoptosis induce by Ad GFP AsHSP70 and expression of HSP70 gene. RESULTS: HSP70 expression of Hep 2 cells was blocked by antisense HSP70 RNA. Typical apoptosis cells were seen in experimental group. It was verified by immuohistochemistry that only low expression of HSP70 or no expression in the antisense group, compared with control group. FCM analysis showed that subploidy peaks were found in the antisense group but not in control group. CONCLUSION: The results indicate that HSP70 antisense RNA plays an important biological role in laryngeal cancer cells and can possibly be a new method for laryngeal cancer gene therapy.